The Mouse Brain Atlas provides a more extended overview of the brain proteome. Extended tissue samples include mouse brain, human lactating breast, eye, and additional samples of adrenal gland, skin and brain. In addition to the standard tissue setup, extended tissue profiling is performed for selected proteins, to give a more complete overview on where the protein is expressed. The Mouse Brain Atlas (under development) Selective cytoplasmic expression in cardiomyocytes at the protein level, highly tissue enriched in heart muscle at the mRNA level. For each gene, the immunohistochemical staining profile is matched with mRNA data and gene/protein characterization data to yield an "annotated protein expression" profile. The antibody-based protein profiles are qualitative and describe the spatial distribution, cell type specificity and the rough relative abundance of proteins in these tissues, whereas the mRNA data provide quantitative data on the average gene expression within an entire tissue. Altogether 76 different cell types, corresponding to 44 normal human tissue types covering all major parts of the human body, have been analyzed manually and the data is presented as histology-based annotation of protein expression levels. The Tissue Atlas shows the expression and localization of human proteins across tissues and organs, based on deep sequencing of RNA (RNA-seq) from 37 major different normal tissue types and immunohistochemistry on tissue microarrays containing 44 different tissue types. The full publication list is available here. A pathology atlas of the human cancer transcriptome. ![]() The Human Protein Atlas consortium is funded by the Knut and Alice Wallenberg Foundation. The Human Protein Atlas program has already contributed to several thousands of publications in the field of human biology and disease and it is selected by the organization ELIXIR (as a European core resource due to its fundamental importance for a wider life science community. ![]() The Human Protein Atlas consists of three separate parts, each focusing on a particular aspect of the genome-wide analysis of the human proteins the Tissue Atlas showing the distribution of the proteins across all major tissues and organs in the human body, the Cell Atlas showing the subcellular localization of proteins in single cells, and finally the Pathology Atlas showing the impact of protein levels for survival of patients with cancer. All the data in the knowledge resource is open access to allow scientists both in academia and industry to freely access the data for exploration of the human proteome. The Human Protein Atlas is a Swedish-based program initiated in 2003 with the aim to map all the human proteins in cells, tissues and organs using integration of various omics technologies, including antibody-based imaging, mass spectrometry-based proteomics, transcriptomics and systems biology.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |